| Customization: | Available |
|---|---|
| After-sales Service: | 1 Year |
| Warranty: | 1 Year |
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Description
A large number of examples show that bacteria can penetrate shielding materials along with dry organic or inorganic particles, such as skin flakes or clean suits carrying bacteria, or particles carrying bacteria penetrating packaging materials during storage. This instrument can be used to determine the bacteria penetration performance of materials against dry particles within the size range of human skin flakes.
Test Principle
The test is conducted on specimens fixed on a container. In these containers, five containers with talcum powder carrying Bacillus subtilis and one container with talcum powder without bacteria as a control are used. A Petri dish is inserted close to the bottom of each container below the specimen. The equipment supporting the containers is oscillated by a gas ball oscillator, and all the talcum powder penetrating the specimens falls onto the Petri dishes. The Petri dishes are taken out and cultured. The number of colonies grown is counted.
Execution Standard
According to ISO 22612, YY/T0506.5-2009, YY/T0471
ISO 22612 Dry Microbial Penetration Resistence Test Equipment Bacteria Penetration Resistance Tester
Technical Specifications
| Control System | PLC |
| Operation Interface | 7-inch color touch screen with Chinese and English switching function |
| Test Chamber | Equipped with built-in exhaust fan, built-in filter, lighting lamp and sterilizing lamp |
| Test Containers | The instrument is equipped with 6 stainless steel test containers, each with a metal piston on the top. The metal piston can be inserted through the central hole of the lid to a position 10mm below the lid, and the test piece shall be kept non-loose after insertion. Meanwhile, each container is provided with a slot at the bottom accessory, which is used for inserting a petri dish. |
| Pneumatic Ball Oscillator | The external air source passes through the pneumatic ball oscillator, and the vibration frequency is maintained at the target frequency after corresponding adjustment and calibration. It can generate 20,800 vibrations per minute with a force of 650N. |
| Marble Plate | A marble plate with an area of 400mm×400mm and a thickness of 10mm is used as the operating platform of the entire instrument. |
| Flow Meter | Mainly used to measure the air flow that generates a vibration frequency of 20,800 times per minute (347Hz) |
| Test Specimen Size | 200mm×200mm |
| Instrument Air Source | 0.8MPa compressed air |
| Noise Level | Approximately 90dB |
| Instrument Dimensions | 950mm×600mm×650mm |
| Instrument Weight | 85kg |
| Power Supply | 220V |
Configuration List
1. One main unit;
2. Six test boxes;
3. One power cord;
4. One instruction manual;
5. Optional accessories as needed:
6. Petri dish: 90mm, 6 pieces
Analysis of Test Steps
1. Sample Preparation:
(1) Cut 12 test pieces (12 pieces).
(2) Sterilization Treatment
Sterilize the cut test pieces and each test container.
(3) Fix the Test Container
Fix the test container onto the fixed plate using a rubber ring, ensuring a tight connection with the fixed plate and placing the bottom against the stone plate.
(4) Talcum Powder Preparation
Prepare talcum powder containing AATCC 9372 Bacillus subtilis spores, with a concentration of approximately 108 CFU/g. Refer to YY/T 5056.5-2009 for the preparation process.
2. Start the Test.
Remove the test pieces using aseptic operation and place them on the mouths of each test container.
Push the piston downward to fix the cover onto the container, ensuring the test pieces are fixed under controlled relaxation conditions.
Remove the piston. Add 0.5g ± 0.1g of contaminated talcum powder to the test piece, with the sixth container adding uninfected talcum powder as a control. Seal the piston opening with a film.
Put a small plastic bag over each container.
Insert the uncovered petri dish through the narrow opening at the bottom of each container. Seal the opening with adhesive tape.
Vibrate at a frequency of 20800 times per minute for 30 minutes.
Remove the plastic bag and adhesive tape.
Insert the cover of the petri dish through the opening and remove the petri dish. Cultivate it at 35°C for 24 hours.
Count the formed colonies. The valid sample should have a reading of 0 for the control dish. Otherwise, it indicates external contamination and the test should be stopped.
Repeat the above test steps.
Test at least 2 groups. Calculate the arithmetic mean of 10 valid results as the final result.
Test Completion
When the test is completed, ensure the data has been recorded. Adjust the pressure knob on the control panel to the 0 mark. Turn off the air intake device of the instrument, turn on the exhaust fan and lighting, and clean the surface of each sample box.
3. Maintenance and Care
Keep the instrument dry
To ensure the normal operation of the instrument and extend its service life, keep the instrument dry. Use it in a relatively dry environment and avoid water droplets splashing or condensation, as it may damage non-water-resistant components. If the instrument is not used for a long time, disconnect the power supply and cut off the air source.
Component Damage
The internal oscillator of the instrument is an easily damaged component. Once it reaches the end of its service life, the accuracy of the oscillation frequency will decrease. It is recommended to replace the new component at this time.
Also, if the marble plate is damaged, replace it with the same specification part in time.
Microbial Safety
To prevent contamination of the test personnel and the test environment, the test personnel should have the operational basis for microbial cultivation. Follow the laboratory operation procedures strictly.
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